Journal of Systems Engineering and Electronics ›› 2025, Vol. 36 ›› Issue (6): 1658-1664.doi: 10.23919/JSEE.2025.000086

• CONTROL THEORY AND APPLICATION • Previous Articles    

Miniaturized two-photon microscopy system for extreme shock and vibration environment

Bosong YU1(), Junjie WANG2(), Yizhou LIU3,4(), Conghao WANG2(), Honghao MA1(), Lishuang FENG1,5,*(), Aimin WANG6()   

  1. 1 School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China
    2 College of Future Technology, Peking University, Beijing, 100871, China
    3 School of Information Science and Engineering, and Shandong Provincial Key Laboratory of Laser Technology and Application, Shandong University, Qingdao 266237, China
    4 Key Laboratory of Laser & Infrared System Ministry of Education, Shandong University, Qingdao 266237, China
    5 Key Laboratory of Precision Opto-Mechatronics Technology (Ministry of Education), Beihang University, Beijing 100191, China
    6 School of Electronics, Peking University, Beijing 100871, China
  • Received:2025-04-18 Online:2025-12-18 Published:2026-01-07
  • Contact: Lishuang FENG E-mail:bosongyu@buaa.edu.cn;Junjie_wang@pku.edu.cn;yizhou.liu@sdu.edu.cn;chwang24@pku.edu.cn;mahonghao@buaa.edu.cn;fenglishuang@buaa.edu.cn;wangaimin@pku.edu.cn
  • About author:
    YU Bosong was born in 1994. He received his M.S. degree from Huaqiao University, Xiamen, China, in 2020. He is currently a Ph.D. candidate in the School of Instrumentation and Optoelectronic Engineering, Beihang University. His research interests are fiber femtosecond lasers and multiphoton microscopy. E-mail: bosongyu@buaa.edu.cn

    WANG Junjie was born in 1987. He received his Ph.D. degree from Beihang University in 2016. Now he is a postdoc fellow in Peking University. His research interests include biomedical imaging and multiphoton microscopy. E-mail: Junjie_wang@pku.edu.cn

    LIU Yizhou was born in 1990. He received his Ph.D. degree from the Department of Physics at the University of Hamburg, Germany, in 2019. From 2019 to 2020, he conducted postdoctoral research at the Deutsches Elektronen-Synchrotron (DESY) Free Electron Laser Center in Germany. Now he is an associate professor in the School of Information Science and Engineering, Shandong University. His research interests include high-power fiber laser and high-energy solid-state laser. E-mail: yizhou.liu@sdu.edu.cn

    WANG Conghao was born in 1996. He received his Ph.D. degree from Beihang University, Beijing, China, in 2024. He is a postdoctoral researcher in Peking University. His research interests include two-photon endomicroscopic imaging. E-mail: chwang24@pku.edu.cn

    MA Honghao was born in 1992. He received his Ph.D. degree from the School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing, China, in 2022. Now he is a lecturer in the School of Instrumentation and Optoelectronic Engineering, Beihang University. His research interests include resonant fiber optic gyros. E-mail: mahonghao@buaa.edu.cn

    FENG Lishuang was born in 1968. She received her Ph.D. degree from the Saint Petersburg Institute of Fine Mechanics and Optics, Russia, in 1996. From 1997 to 2001, she was an associate professor with the School of Photoelec-tronic Information and Communication Engineering, Beijing Information Science and Technology University. In 2001, she joined the School of Instrumentation and Optoelectronic Engineering, Beihang University, where she is currently a professor. Her research interests include integrated optics and micro-opto-electro-mechanical (MOEMS) systems, advanced optical sensors, and optoelectronics devices. E-mail: fenglishuang@buaa.edu.cn

    WANG Aimin was born in 1978. He received his Ph.D. degree from University of Bath, U.K, in 2007. He is currently a tenured associate professor at the School of Electronics, Peking University. His research interests include biomedical imaging and multiphoton microscopy. E-mail: wangaimin@pku.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (62475008; 62305186).

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Abstract:

Two-photon fluorescence microscopy, based on the principles of two-photon excited fluorescence and second harmonic generation, enables real-time non-invasive in vivo imaging of skin and cells, providing a means to assess human health status. In this paper, a miniaturized two-photon imaging system is designed and fabricated to withstand extreme vibration and shock environments. The mechanical stability of the optical and structural components of the miniaturized probe is evaluated under random vibration and shock vibration tests using finite element simulation methods and ray tracing techniques. During the environmental testing, the maximum stress on the probe is 11.5 MPa, which is well below the threshold for structural failure. The largest structural displacement occurs at the collimator, where random vibrations produce an offset of 10.9 μm. This offset is analyzed by using geometric optics and point spread functions. Under the maximum collimator offset, the theoretical resolution, as calculated by the point spread function, shifted from 463.28 nm to 463.48 nm. Additionally, a lateral offset of 127 nm is observed at the center position, which does not significantly impact the imaging performance. Finally, environmental and imaging performance tests are conducted. The system’s measured resolution after the environmental tests is 530 nm, consistent with its resolution prior to testing. Imaging tests are also performed on the skin’s stratum corneum, granular layer, spinous layer, and basal cell layer, revealing clear cellular structural information. These results confirm the device’s potential for applications in extreme shock and vibration environments.

Key words: miniaturized two-photon microscopy, finite element simulation, geometric optics simulation

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